Alessandra Scelsi, Brigida Bochicchio* and Antonietta Pepe* Pages 319 - 325 ( 7 )
Background: The conjugation of small organic molecules to self-assembling peptides is a versatile tool to decorate nanostructures with original functionalities. Labeling with chromophores or fluorophores, for example, creates optically active fibers with potential interest in photonic devices.
Aim and Objective: In this work, we present a rapid and effective labeling procedure for a self-assembling peptide able to form nanofibers. Rapid periodate oxidation of the N-terminal serine residue of the peptide and subsequent conjugation with dansyl moiety generated fluorophore-decorated peptides.
Results: Three dansyl-conjugated self-assembling peptides with variable spacer-length were synthesized and characterized and the role of the size of the linker between fluorophore and peptide in self-assembling was investigated. Our results show that a short linker can alter the self-assembly in nanofibers of the peptide.
Conclusions: Herein we report on an alternative strategy for creating functionalized nanofibrils, able to expand the toolkit of chemoselective bioconjugation strategies to be used in site-specific decoration of self-assembling peptides.
Bioconjugation, peptide, periodate oxidation, self-assembly, fluorescent tag, atomic force microscopy.
Department of Science, University of Basilicata, Potenza, Department of Science, University of Basilicata, Potenza, Department of Science, University of Basilicata, Potenza