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Molecular Probes for Bioluminescence Imaging

[ Vol. 8 , Issue. 4 ]

Author(s):

Song Wu, Edwin Chang and Zhen Cheng   Pages 488 - 497 ( 10 )

Abstract:


Bioluminescence refers to the emission of light from a living system in which photoproteins such as luciferase enzymes oxidize their substrates to produce light. Because of its high-sensitivity and low-toxicity, bioluminescence imaging (BLI) is particularly useful for in vitro assays and in vivo small animal imaging. It provides a powerful tool to study various important biological questions and processes including gene and protein expression, protein-protein interactions, protein-nucleic acid interactions, and cell signaling pathway functions. This review highlights some of the latest developments in the design and applications of molecular probes for BLI.

Keywords:

Bioluminescence imaging, Molecular Imaging, Probe, Luciferase, Bioluminescence Resonance Energy Transfer, MRI, Luciferase Mutugenesis, D-luciferin Analogs, Luciferase Fused Proteins, Intramolecular Enzyme Complementation

Affiliation:

Molecular Imaging Program at Stanford, Department of Radiology, 1201 Welch Road, Lucas Expansion, P095, Stanford University, Stanford, CA 94305, USA.



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